cytation 5 cell imaging multimode reader biotek Search Results


96
BMG Labtech biotek cytation 5 cell imaging multimode reader
Biotek Cytation 5 Cell Imaging Multimode Reader, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BioTex Inc cytation 5 cell imaging multimode reader
Cytation 5 Cell Imaging Multimode Reader, supplied by BioTex Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Ogd, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Protein Sciences Inc cell imaging multimode reader
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Cell Imaging Multimode Reader, supplied by Protein Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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Promega luciferase assay reagent
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Luciferase Assay Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sony imx264 cmos camera
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Imx264 Cmos Camera, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Hamamatsu nanozoomer 2.0-ht system
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Nanozoomer 2.0 Ht System, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega luciferin substrate
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Luciferin Substrate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega caspase-glos 3/7 assay system
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Caspase Glos 3/7 Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega one-glo luciferase assay system
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
One Glo Luciferase Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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96
AMS Biotechnology oris cell migration assay kit
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Oris Cell Migration Assay Kit, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Tecan Systems spark multimode microplate reader
A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no <t>OGD</t> or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Spark Multimode Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no OGD or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Monosialoganglioside‐Containing Nanoliposomes Protect Against Acute and Chronic Ischemic Stroke Injury

doi: 10.1161/JAHA.125.042974

Figure Lengend Snippet: A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no OGD or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.

Article Snippet: To confirm that NLGM1 given after hypoxic injury results in upregulation of HO‐1, we exposed a coculture of SH‐SY5Y neuroblastoma (passages 10–13) and human brain microvascular endothelial cells (HBMVECs, passages 7–12) in transwell plates (ThinCert Tissue Culture Inserts; Greiner Bio‐One, Monroe, NC) to either room air with regular media (Complete Classic Media catalog number 4Z0‐500; Cell Systems, Kirkland, WA) for 20 hours or to 3 hours of OGD (1% oxygen, 5% carbon dioxide in a Cytation 5 Cell Imaging Multimode Reader; Biotek, Winooski, VT and DMEM media with no glucose, no glutamine, no phenol red catalog number A1443001; Gibco, Waltham, MA) followed by 17 hours of room air and regular media.

Techniques: MTT Assay, Expressing, Control, Comparison, Transformation Assay