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Thermo Fisher
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Protein Sciences Inc
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Promega
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Image Search Results
Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Monosialoganglioside‐Containing Nanoliposomes Protect Against Acute and Chronic Ischemic Stroke Injury
doi: 10.1161/JAHA.125.042974
Figure Lengend Snippet: A and B , Viability after 20 hours of HBMVECs and SH‐SY5Y cells exposed to no OGD or 3‐hour OGD condition using the MTT assay. OGD condition in both cell types resulted in reduced cell viability compared with no OGD, but the injury was mitigated by treatment with NLGM1 (300 μg/mL) 15 minutes following exposure to OGD. C through G , HBMVECs and SH‐SY5Y cells exposed to no OGD and OGD conditions show that treatment with NLGM1 15 minutes following OGD exposure resulted in increased gene and protein expression of HO‐1 relative to no OGD control in both cell types 20 hours following initiation of OGD. *Statistical analyses comparison was performed for log‐normal‐transformed data. A.U. indicates arbitrary units; HBMVECs, human brain microvascular endothelial cells; HO‐1, heme oxygenase‐1; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; NLGM1, nanoliposomes composed of monosialoganglioside, phosphatidylcholine, and cholesterol; and OGD, oxygen–glucose deprivation.
Article Snippet: To confirm that NLGM1 given after hypoxic injury results in upregulation of HO‐1, we exposed a coculture of SH‐SY5Y neuroblastoma (passages 10–13) and human brain microvascular endothelial cells (HBMVECs, passages 7–12) in transwell plates (ThinCert Tissue Culture Inserts; Greiner Bio‐One, Monroe, NC) to either room air with regular media (Complete Classic Media catalog number 4Z0‐500; Cell Systems, Kirkland, WA) for 20 hours or to 3 hours of
Techniques: MTT Assay, Expressing, Control, Comparison, Transformation Assay